CRL-1576 293 人胚腎細(xì)胞

ATCC® Number: CRL-1576™

Designations: 12MBr6

Depositors: JL Caputo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: epithelial

CRL-1576 293 人胚腎細(xì)胞

Source: Organ: lung

Tissue: bronchus

Disease: normal

Cell Type: epithelial

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Isolation: Isolation date: July, 1979

Receptors: epidermal growth factor （EGF）

Cytogenetic Analysis: modal number = 62; range = 43 to 64.

The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del（1）9 ?; M2 del（13） and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.

Age: 2 to 3 years

Gender: male

Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor （EGF） was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.

Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide （CO2）, 5%

Temperature: 37.0°C

Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.

Subculturing: Protocol: .

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.